Taxodium hybrid ‘zhong shanshan’ research team of plant ecology research center have made a provisionality progress in the molecule mechanism of ethylene response factor of Taxodium hybrid ‘zhong shanshan’ in response to flooding stress. Related research papers were published in 《Molecular Plant Breeding》and《PeerJ》.
     ERF(ethylene response factor) has been proven as the main transcription factors to regulate the genes related to hypoxia and waterlogging stress. In our study， four ThERF genes with significant differences were screened and cloned from the transcriptome data acquired from Taxodium hybrid ‘Zhongshanshan 406’ in short term (1 h) and long term (90 d) waterlogging stress: ThRap2.1（1048bp）（GenBank:KY463469），ThRap2.3（1434bp）（GenBank:KY463470），ThERF15（1060bp）（GenBank:KY463467），ThERF39（1747bp）（GenBank:KY463468）。Multiple protein sequence alignments indicated that only ThRap2.1 and ThRap2.3 proteins had MCGGAI/L domain and belonged to ⅦERF subfamily，and ThRap2.1 sequence is highly homologous with Rap2.12 of Arabidopsis and Sub1A of Oryza sativa and may have functional relationship with waterlogging tolerance. Transient gene expression assay demonstrated that ThERF15, ThERF39 ThRAP2.3 and ThRAP2.1 were all localized to the nucleus. Real-time quantitative PCR revealed that the expression of ThERF15, ThERF39 ThRap2.1 and ThRap2.3 had caused significant increased under waterlogging stress, and in the root is higher than other organs. The change of ethylene content in different tissues and organs was consistent with that of expression of ThRap2.3 gene of T406.
      Article link：http://kns.cnki.net/kcms/detail/46.1068.S.20170914.0848.004.html
           https://peerj.com/articles/4434.html
      This work was supported by the Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, JiangSu Provinces and Chinese Academy of Sciences (QD201401), Natural Science Foundation of Jiangsu Province (No. BK20150551), National Natural Science Foundation of China (No. 31570593). 

Fig. 1 Phylogenetic analysis of the ERF family.

Figure2. Subcellular localization of ThERFs in Populus protoplasts.